The Influence of Temperature on Coumarin 153 Fluorescence Kinetics
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چکیده
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15 صفحه اولThe complex of apomyoglobin with the fluorescent dye coumarin 153.
Understanding a protein's dielectric response requires both a theoretical model and a well-defined experimental system. The former has already been proposed by Song (J. Chem. Phys. 116, 9359 [2002]). We suggest that the latter is provided by the complex of coumarin 153 (C153) with apomyoglobin (ApoMb). C153 has been exhaustively studied and has proven to be an excellent probe of the solvation d...
متن کاملEffects of Matrix Temperature and Rigidity on the Electronic Properties of Solvatochromic Molecules: Electroabsorption of Coumarin 153
Using Stark effect (electroabsorption) spectroscopy to study the well-known solvatochromic probe molecule coumarin 153 (C153) in a variety of polymer matrices and organic glasses, we have found that the average change in polarizability (∆R) that we measure depends critically on the rigidity of the matrix used. In rigid polymer and frozen organic glass matrices, the measured values ∆R are betwee...
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ژورنال
عنوان ژورنال: Journal of Fluorescence
سال: 2011
ISSN: 1053-0509,1573-4994
DOI: 10.1007/s10895-011-0843-1